Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus
نویسندگان
چکیده
Sugarcane yellow leaf virus (SCYLV) is an aphid-transmitted for which Melanaphis sacchari the main vector. Almost all sugarcane varieties grown in Florida are susceptible to SCYLV infection. In this study, we investigated prevalence of accessions Sorghum bicolor another natural host virus. Two field experiments, one 2016 with 19 sorghum lines and other 2017 15 lines, were established at Belle Glade, FL. Stalks collected randomly planted ratoon crops tested by tissue-blot immunoassay (TBIA) reverse transcription-polymerase chain reaction (RT-PCR). Over two-year period, 366 423 S. samples positive TBIA but was detected RT-PCR only 12 161 selected subsamples. Full genome sequences SCYLV, mosaic (SCMV) a new marafivirus obtained high-throughput sequencing (HTS) from three samples. HTS data viruses confirmed RT-PCR. The SCMV isolates appeared be strain species. Positive using antibodies could not systematically associated plant infection or This suggested occurrence non-specific serological unknown antigen. also almum, suggesting that weed reservoir bicolor-infecting Florida.
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ژورنال
عنوان ژورنال: Crop Protection
سال: 2021
ISSN: ['1873-6904', '0261-2194']
DOI: https://doi.org/10.1016/j.cropro.2020.105373